目的 对疏叶卷柏(Selaginella remotifolia Spring) 进行植物化学研究。方法 应用聚酰胺、Sephadex LH-20柱色谱以及半制备型HPLC等现代色谱技术对疏叶卷柏植物的化学成分进行分离纯化,应用质谱和核磁共振技术并结合理化性质对分离得到的 14 个化合物的结构进行鉴定。结果 14 个化合物的结构分别为:罗伯斯塔黄酮(1),4′-甲氧基罗伯斯塔黄酮(2),(2″S)-2″,3″-二氢-4′-甲氧基-罗伯斯塔黄酮(3),(2S,2″S)-四氢罗伯斯塔黄酮(4),穗花杉黄酮(5),7,4′-二甲氧基-穗花杉黄酮(6),4′,7″-二甲氧基-穗花杉黄酮(7),(2″S)-2″,3″-二氢穗花杉黄酮(8),(2″S)-2″,3″-二氢-4′-甲氧基-穗花杉黄酮(9),(2S)-2,3-二氢穗花杉黄酮(10),(2S)-2,3-二氢-4′-甲氧基-穗花杉黄酮(11),(2S)-2,3-二氢-4-甲氧基-穗花杉黄酮(12),chamaeflavone A(13)和8-8″-二聚山柰酚(14)。结论 所有化合物都是首次从该植物中分离得到。其中化合物13,14是首次从该属植物中分离得到。
Abstract
OBJECTIVE To study the chemical constituents of Selaginella remotifolia Spring. METHODS Fourteen biflavonoids were isolated from S. remotifolia by polyamide, Sephadex LH-20 column chromatography and/or Pre-HPLC. RESULTS Their structures were identified by physiochemical properties, MS, and NMR as robustaflavone(1), robustaflavone-4′-methyl ether(2),(2″S)-2″, 3″-dihydrorobustaflavone-4′-methyl ether(3),(2S, 2″S)-tetrahydrorobustaflavone(4), amentoflavone(5), ametoflavone-7, 4′-dimethyl ether(6), amentoflavone-4′, 7″-dimethyl ether(7),(2″S)-2″, 3″-dihydroamentoflavone(8),(2″S)-2″, 3″-dihydroamentoflavone-4′-methyl ether(9),(2S)-2, 3-dihydroamentoflavone(10),(2S)-2, 3-dihydroamentoflavone-4′-methyl ether(11),(2S)-2, 3-dihydroamentoflavone-4-methyl ether(12), chamaeflavone A(13), and 8-8″-bi-kaempferol(14) . CONCLUSION All compounds were isolated from this plant for the first time and compounds 13 and 14 are isolated from this genus for the first time.
关键词
卷柏科 /
疏叶卷柏 /
双黄酮 /
chamaeflavone A /
8-8″-二聚山柰酚
{{custom_keyword}} /
Key words
Selaginellaceae /
Selaginella remotifolia /
biflavonoid /
chamaeflavone A /
8-8″-bi-kaempferol
{{custom_keyword}} /
中图分类号:
R284.1
{{custom_clc.code}}
({{custom_clc.text}})
{{custom_sec.title}}
{{custom_sec.title}}
{{custom_sec.content}}
参考文献
[1] Jiangsu New Medical College. A Dictionary of Traditional Chinese Drugs(中药大辞典上册) . Shanghai:Shanghai Science & Technology Press, 1985:1472.
[2] ZHEN X K, NING T L, WANG X L, et al. Effects of total flavonoids and amntoflavone isolated from Selaginella tamariscina on human umbilical vein endothelial cells proliferation and VEGF expression. Chin Pharm J(中国药学杂志), 2011, 46(13):998-1002.
[3] ZHANG H W, SUN X F, TIAN J K. Research overview of flavonoids from Selaginella . Asia-Pacific Trad Med (亚太传统医药), 2007, 3(10):63-65.
[4] JIANG X P, CHEN K L. Study on biflavonoids from Selaginella moellendorfii Hieron . Chin Pharm J(中国药学杂志), 2009, 44(2):96-98.
[5] XU K P, SHEN J, LIU J F, et al. Studies on two characteristic constituents in four species of herbs Selaginella . Chin Pharm J(中国药学杂志), 2009, 44(20):1535-1539.
[6] ZHENG J, WANG N, FAN M, et al. A new bifavonoid from Selaginella uncinata . Asian J Trad Med, 2007, 2(3):92-97.
[7] LIU P P, LIU Y, LIU Y G, et al. Preparation method of chemical reference amentoflavone from Selaginella tamariscina (Beauv.) Spring. China Pharm (中国药业), 2012, 21(13):12-13.
[8] ZOU Z X, XU K P, ZOU H, et al. Biflavonoids from Selaginella moellendor Hieron. Cent South Pharm (中南药学), 2012, 10(1):4-6.
[9] SILVA G L, CHAI H, GUPTA M P, et al. Cytotoxic biflavonoids from Selaginella willdenovii . Phytochemistry, 1995, 40(1):129-134.
SWAMY R C, KUNERT O, SCHUHLY W, et al. Structurally unique biflavonoids from Selaginella chrysocaulos and Selaginella bryopteris . Chem Biodiver, 2006, 3(4):405-413.
ZHENG J X, ZHENG Y, ZHI H, et al. New 3′, 8″-Linked biflavonoids from Selaginella uncinata displaying protective effect against anoxia . Molecules, 2011, 16(8):6206-6214.
LIU H Q, LIN R C, MA S C, et al. Studies on chemical constituents of Selaginella stauntoniana(I) . Chin Trad Herb Drugs(中草药), 2003, 34(4):298-299.
DAS B, MAHENDER G, RAO K Y, et al. Biflavonoids from Cycas beddomei . Chem Pharm Bull, 2005, 53(1):135-136.
ASADA Y, SUKEMORI A, WATANABE T, et al. Isolation, structure determination, and anti-HIV evaluation of tigliane-type diterpenes and biflavonoid from Stellera chamaejasme . J Nat Prod, 2013, 76(5):852-857.
YE Y, GUO Y, LUO Y T, et al. Isolation and free radical scavenging activities of a novel biflavonoid from the shells of Camellia oleifera Abel . Fitoterapia, 2012, 83(8):1585-1589.
{{custom_fnGroup.title_cn}}
脚注
{{custom_fn.content}}